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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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0
Conjugate
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验证数据展示

  • WB analysis using 83187-3-RR (same clone as 83187-3-PBS)

    WB analysis using 83187-3-RR (same clone as 83187-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 83187-3-RR (same clone as 83187-3-PBS)

    WB analysis of human testis using 83187-3-RR (same clone as 83187-3-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

    IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83187-3-RR (same clone as 83187-3-PBS)

    FC experiment of A431 using 83187-3-RR (same clone as 83187-3-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SMC6 Recombinant antibody (83187-3-RR, Clone:240015F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

    IF Staining of mouse testis using 83187-3-RR (same clone as 83187-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83187-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00255-2

    Cytometric bead array standard curve of MP00255-2

    Cytometric bead array standard curve of MP00255-2, SMC6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83187-3-PBS. Detection antibody: 83187-1-PBS. Standard: Ag6898. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83187-3-RR

    Affinity and Kinetic Characterization of 83187-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83187-3-RR against Human SMC6 were performed. The affinity constant is 0.428 nM.

SMC6 Recombinant antibody, PBS Only (Capture)

SMC6 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83187-3-PBS

产品说明书

CloneNo. 240015F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83187-3-PBS targets SMC6 as part of a matched antibody pair:

MP00255-2: 83187-3-PBS capture and 83187-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SMC6 fusion protein Ag6898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 structural maintenance of chromosomes 6
别名 SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6
计算分子量 126 kDa
观测分子量 130 kDa
GenBank蛋白编号BC039828
基因名称 SMC6
Gene ID (NCBI) 79677
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96SB8
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).