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验证数据展示

  • IF Staining of mouse testis using CL488-67808

    IF Staining of mouse testis using CL488-67808

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 SMCR7L antibody (CL488-67808, Clone: 3B3G3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SMCR7L/MID51 Monoclonal antibody

SMCR7L/MID51 Monoclonal Antibody for IF-P
Cat No. CL488-67808

产品说明书

CloneNo. 3B3G3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF-P

别名

SMCR7L, Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like, SMCR7-like protein, MIEF1, MID51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 67808-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67808 targets SMCR7L/MID51 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 SMCR7L/MID51 fusion protein Ag13775 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Smith-Magenis syndrome chromosome region, candidate 7-like
别名 SMCR7L, Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like, SMCR7-like protein, MIEF1, MID51
计算分子量 463 aa, 51 kDa
观测分子量48-51 kDa
GenBank蛋白编号BC002587
基因名称 SMCR7L
Gene ID (NCBI) 54471
RRIDAB_2919552
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQG6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SMCR7L/MID51 antibody CL488-67808Download protocol
Standard Protocols
Click here to view our Standard Protocols