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  • Featured Product
  • KD/KO Validated

SMN (Human,Mouse,Rat) Monoclonal antibody, PBS Only

SMN (Human,Mouse,Rat) Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3C10B5

Cat No : 60154-2-PBS

Print datasheet

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN (Human,Mouse,Rat), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

验证数据展示

  • WB analysis of HeLa using 60154-2-Ig (same clone as 60154-2-PBS)

    WB analysis of HeLa using 60154-2-Ig (same clone as 60154-2-PBS)

    WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

  • WB analysis using 60154-2-Ig (same clone as 60154-2-PBS)

    WB analysis using 60154-2-Ig (same clone as 60154-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

    IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

    IHC staining of mouse liver using 60154-2-Ig (same clone as 60154-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60154-2-Ig (same clone as 60154-2-PBS)

    IF Staining of HepG2 using 60154-2-Ig (same clone as 60154-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60154-2-PBS in a different storage buffer formulation.

产品信息

60154-2-PBS targets SMN (Human,Mouse,Rat) in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen SMN (Human,Mouse,Rat) fusion protein Ag14333 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name survival of motor neuron 2, centromeric
Synonyms C BCD541, Component of gems 1, Gemin 1, SMN, SMN (Human,Mouse,Rat), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein
Calculated Molecular Weight 282 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000908
Gene Symbol SMN2
Gene ID (NCBI) 6607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.