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- KD/KO Validated
SMN Polyclonal antibody
SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, mouse testis tissue, HepG2 cells, Jurkat cells, K-562 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11708-1-AP targets SMN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP, ELISA |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SMN fusion protein Ag2260 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | survival of motor neuron 2, centromeric |
Synonyms | SMN1,SMN, SMN1, Gemin-1, Gemin 1, Component of gems 1 |
Calculated Molecular Weight | 282 aa, 30 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC000908 |
Gene Symbol | SMN |
Gene ID (NCBI) | 6607 |
RRID | AB_2255114 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16637 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMN antibody 11708-1-AP | Download protocol |
IHC protocol for SMN antibody 11708-1-AP | Download protocol |
IF protocol for SMN antibody 11708-1-AP | Download protocol |
IP protocol for SMN antibody 11708-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation | ||
Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice | ||
Hum Mol Genet SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA. | ||
Hum Mol Genet Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA. | ||
Neurobiol Dis Dual SMN inducing therapies can rescue survival and motor unit function in symptomatic ∆7SMA mice. | ||
Hum Mol Genet Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.
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