- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated SMN Polyclonal antibody
SMN Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 11708-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11708 targets SMN in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | SMN fusion protein Ag2260 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | survival of motor neuron 2, centromeric |
| Synonyms | C BCD541, Component of gems 1, FLJ76644, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein |
| Calculated Molecular Weight | 282 aa, 30 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC000908 |
| Gene Symbol | SMN2 |
| Gene ID (NCBI) | 6607 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16637 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SMN antibody CL488-11708 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |