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  • Featured Product
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SMN (Human-Specific) Monoclonal antibody

SMN (Human-Specific) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

2C6D9

Cat no : 60154-1-Ig

Print datasheet

Synonyms

SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541

验证数据展示

  • WB analysis of A375 using 60154-1-Ig

    WB analysis of A375 using 60154-1-Ig

    A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 60154-1-Ig

    WB analysis of HEK-293 using 60154-1-Ig

    WB result of SMN (Human-Specific) antibody (60154-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human-Specific) transfected HEK-293 cells.

  • IHC staining of human brain using 60154-1-Ig

    IHC staining of human brain using 60154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HEK-293 using 60154-1-Ig

    IP experiment of HEK-293 using 60154-1-Ig

    IP result of anti-SMN (Human-Specific) (IP:60154-1-Ig, 4ug; Detection:60154-1-Ig 1:500) with HEK-293 cells lysate 2440ug.

  • IF Staining of HepG2 using 60154-1-Ig

    IF Staining of HepG2 using 60154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human-Specific) antibody (60154-1-Ig, Clone: 2C6D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 60154-1-Ig

    FC experiment of Jurkat using 60154-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SMN (Human-Specific) Monoclonal antibody (60154-1-Ig, Clone: 2C6D9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA375 cells, Raji cells, HepG2 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human heart tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60154-1-Ig targets SMN (Human-Specific) in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SMN (Human-Specific) fusion protein Ag14333 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name survival of motor neuron 2, centromeric
Synonyms SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541
Calculated Molecular Weight 282 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000908
Gene Symbol SMN
Gene ID (NCBI) 6607
RRIDAB_10639513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.

实验方案

Product Specific Protocols
WB protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IHC protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IF protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
IP protocol for SMN (Human-Specific) antibody 60154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Chem Biol

SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice.

Authors - James Palacino
View Article
humanWB

Science

Base editing rescue of spinal muscular atrophy in cells and in mice

Authors - Mandana Arbab
View Article