验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 60154-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60154 targets SMN (Human,Mouse,Rat) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SMN (Human,Mouse,Rat) fusion protein Ag14333 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | survival of motor neuron 2, centromeric |
| 别名 | SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541 |
| 计算分子量 | 282 aa, 30 kDa |
| GenBank蛋白编号 | BC000908 |
| 基因名称 | SMN |
| Gene ID (NCBI) | 6607 |
| RRID | AB_2919231 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16637 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SMN (Human,Mouse,Rat) antibody CL488-60154 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |