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SMPD2 Recombinant antibody

SMPD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240400B5

Cat no : 83520-3-RR

Print datasheet

Synonyms

N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12

验证数据展示

  • WB analysis using 83520-3-RR

    WB analysis using 83520-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83520-3-RR

    FC experiment of U2OS using 83520-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 83520-3-RR

    FC experiment of HeLa using 83520-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83520-3-RR

    Affinity and Kinetic Characterization of 83520-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293T cells, MOLT-4 cells, SH-SY5Y cells
Positive FC (Intra) detected inHeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83520-3-RR is SMPD2 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SMPD2 fusion protein Ag7367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
Synonyms N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC000038
Gene Symbol SMPD2
Gene ID (NCBI) 6610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60906
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).

实验方案

Product Specific Protocols
WB protocol for SMPD2 antibody 83520-3-RRDownload protocol
FC protocol for SMPD2 antibody 83520-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols