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验证数据展示

  • WB analysis of mouse liver using 15511-1-AP

    WB analysis of mouse liver using 15511-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15511-1-AP

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SMU1 antibody included
  • IHC staining of human kidney using 15511-1-AP

    IHC staining of human kidney using 15511-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SMU1 antibody included
  • IF Staining of HepG2 using 15511-1-AP

    IF Staining of HepG2 using 15511-1-AP

    Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SMU1 Polyclonal antibody

SMU1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15511-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

WD40 repeat-containing protein SMU1, Smu-1 suppressor of mec-8 and unc-52 protein homolog, SMU 1, SAP57, fSAP57

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, K-562 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human hepatocirrhosis tissue, human ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMU1 Ready-To-Use IHC Kit. SMU1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15511-1-AP targets SMU1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMU1 fusion protein Ag7688 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
别名 WD40 repeat-containing protein SMU1, Smu-1 suppressor of mec-8 and unc-52 protein homolog, SMU 1, SAP57, fSAP57
计算分子量 58 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC002876
基因名称 SMU1
Gene ID (NCBI) 55234
RRIDAB_2270843
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2TAY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.

实验方案

Product Specific Protocols
WB protocol for SMU1 antibody 15511-1-APDownload protocol
IHC protocol for SMU1 antibody 15511-1-APDownload protocol
IF protocol for SMU1 antibody 15511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols