• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SMURF1 Monoclonal antibody

SMURF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A7E4

Cat no : 68466-1-Ig

Print datasheet

Synonyms

hSMURF1, KIAA1625, SMURF1

验证数据展示

  • WB analysis using 68466-1-Ig

    WB analysis using 68466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68466-1-Ig (SMURF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, A549 cells, U2OS cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68466-1-Ig targets SMURF1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen SMURF1 fusion protein Ag30891 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SMAD specific E3 ubiquitin protein ligase 1
Synonyms hSMURF1, KIAA1625, SMURF1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight86 kDa
GenBank Accession NumberNM_020429
Gene Symbol SMURF1
Gene ID (NCBI) 57154
RRIDAB_3085177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

实验方案

Product Specific Protocols
WB protocol for SMURF1 antibody 68466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols