• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using CL488-66330

    WB analysis using CL488-66330

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66330 (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated SMYD3 Monoclonal antibody

SMYD3 Monoclonal Antibody for WB
Cat No. CL488-66330

产品说明书

CloneNo. 1B5C10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB

别名

bA74P14.1, FLJ21080, SMYD3, ZMYND1, ZNFN3A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66330-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66330 targets SMYD3 in WB applications and shows reactivity with human samples.

经测试应用 WB Application Description
经测试反应性 human
免疫原 SMYD3 fusion protein Ag2624 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET and MYND domain containing 3
别名 bA74P14.1, FLJ21080, SMYD3, ZMYND1, ZNFN3A1
计算分子量428aa,49 kDa; 369aa,42 kDa
观测分子量 49 kDa
GenBank蛋白编号BC031010
基因名称 SMYD3
Gene ID (NCBI) 64754
RRIDAB_3084234
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H7B4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) .

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 SMYD3 antibody CL488-66330Download protocol
Standard Protocols
Click here to view our Standard Protocols