SNAP25 Recombinant antibody
SNAP25 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240069F12
验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells, mouse brain tissue, rat brain tissue |
Positive IF detected in | PC-12 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83259-5-RR is SNAP25 Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, rat, mouse |
Immunogen | SNAP25 fusion protein Ag6695 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | synaptosomal-associated protein, 25kDa |
Synonyms | bA416N4.2, dJ1068F16.2, FLJ23079, RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein |
Calculated Molecular Weight | 23 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC010647 |
Gene Symbol | SNAP25 |
Gene ID (NCBI) | 6616 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P60880 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.
实验方案
Product Specific Protocols | |
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WB protocol for SNAP25 antibody 83259-5-RR | Download protocol |
IF protocol for SNAP25 antibody 83259-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |