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Host
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0
Conjugate
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验证数据展示

  • WB analysis using 14093-1-AP (same clone as 14093-1-PBS)

    WB analysis using 14093-1-AP (same clone as 14093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14093-1-AP (SNAP91 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    IF Staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SNAP91 antibody (14093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

SNAP91 Polyclonal antibody, PBS Only

SNAP91 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 14093-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14093-1-PBS targets SNAP91 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNAP91 fusion protein Ag5224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptosomal-associated protein, 91kDa homolog (mouse)
别名 Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein
计算分子量92.5 kDa
观测分子量160 kDa
GenBank蛋白编号BC060818
基因名称 SNAP91
Gene ID (NCBI) 9892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60641
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).