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验证数据展示

  • WB analysis using 68046-1-Ig

    WB analysis using 68046-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68046-1-Ig (SNCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of mouse brain using 68046-1-Ig

    IF Staining of mouse brain using 68046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SNCB antibody (68046-1-Ig, Clone: 2B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SNCB Monoclonal antibody

SNCB Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68046-1-Ig

产品说明书

CloneNo. 2B10E10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit, chicken

应用

WB, IF-P, ELISA

别名

Beta synuclein, SNCB, synuclein, beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, rabbit brain, rat brain, mouse brain, chicken brain
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68046-1-Ig targets SNCB in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit, chicken
免疫原 SNCB fusion protein Ag0772 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synuclein, beta
别名 Beta synuclein, SNCB, synuclein, beta
计算分子量 14 kDa
观测分子量18 kDa
GenBank蛋白编号BC002902
基因名称 SNCB
Gene ID (NCBI) 6620
RRIDAB_2918788
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16143
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNCB antibody 68046-1-IgDownload protocol
IF protocol for SNCB antibody 68046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols