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验证数据展示

  • FC experiment of SH-SY5Y using CL488-68046

    FC experiment of SH-SY5Y using CL488-68046

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SNCB (CL488-68046, Clone:2B10E10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SNCB Monoclonal antibody

SNCB Monoclonal Antibody for FC (Intra)
Cat No. CL488-68046

产品说明书

CloneNo. 2B10E10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit, chicken

应用

FC (Intra)

别名

Beta synuclein, SNCB, synuclein, beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68046-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68046 targets SNCB in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat, pig, rabbit, chicken
免疫原 SNCB fusion protein Ag0772 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synuclein, beta
别名 Beta synuclein, SNCB, synuclein, beta
计算分子量 14 kDa
观测分子量18 kDa
GenBank蛋白编号BC002902
基因名称 SNCB
Gene ID (NCBI) 6620
RRIDAB_2923869
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16143
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SNCB antibody CL488-68046Download protocol
Standard Protocols
Click here to view our Standard Protocols