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验证数据展示

  • IF Staining of HepG2 using CL594-60265

    IF Staining of HepG2 using CL594-60265

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated SND1 antibody (CL594-60265, Clone: 1A6A4 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SND1 Monoclonal antibody

SND1 Monoclonal Antibody for IF/ICC
Cat No. CL594-60265

Print datasheet

CloneNo. 1A6A4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SND1 Ready-To-Use IHC Kit. SND1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60265-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60265 targets SND1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen SND1 fusion protein Ag1200 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name staphylococcal nuclease and tudor domain containing 1
Synonyms 100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC017180
Gene Symbol SND1
Gene ID (NCBI) 27044
RRIDAB_2919906
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7KZF4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

实验方案

Product Specific Protocols
IF protocol for CL594 SND1 antibody CL594-60265Download protocol
Standard Protocols
Click here to view our Standard Protocols