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SNF8 Monoclonal antibody

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2C10F2

Cat no : 67696-1-Ig

Print datasheet

Synonyms

Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22

验证数据展示

  • WB analysis using 67696-1-Ig

    WB analysis using 67696-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67696-1-Ig

    IHC staining of human breast cancer using 67696-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67696-1-Ig

    IF Staining of HeLa using 67696-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67696-1-Ig targets SNF8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen SNF8 fusion protein Ag30384 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Synonyms Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22
Calculated Molecular Weight 258 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC008976
Gene Symbol SNF8
Gene ID (NCBI) 11267
RRIDAB_2882888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96H20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNF8 antibody 67696-1-IgDownload protocol
IHC protocol for SNF8 antibody 67696-1-IgDownload protocol
IF protocol for SNF8 antibody 67696-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy

Authors - Takashi Nozawa
View Article