SNF8 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10F2

Cat no : 67696-1-PBS

Synonyms

Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22

WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

Size

67696-1-PBS targets SNF8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	SNF8 fusion protein Ag30384 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Synonyms	Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22
Calculated Molecular Weight	258 aa, 29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC008976
Gene Symbol	SNF8
Gene ID (NCBI)	11267
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q96H20
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C