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SNRNP200 Polyclonal antibody

SNRNP200 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 23875-1-AP

Print datasheet

Synonyms

KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1

验证数据展示

  • WB analysis of HeLa using 23875-1-AP

    WB analysis of HeLa using 23875-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 23875-1-AP

    IP experiment of HeLa using 23875-1-AP

    IP result of anti-SNRNP200 (IP:23875-1-AP, 4ug; Detection:23875-1-AP 1:1000) with HeLa cells lysate 4000ug.

  • WB analysis using 23875-1-AP

    WB analysis using 23875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23875-1-AP (SNRNP200 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 23875-1-AP

    IHC staining of mouse testis using 23875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRNP200 antibody included
  • IHC staining of human placenta using 23875-1-AP

    IHC staining of human placenta using 23875-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRNP200 antibody included
  • IHC staining of mouse bladder using 23875-1-AP

    IHC staining of mouse bladder using 23875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRNP200 antibody included
  • IHC staining of rat stomach using 23875-1-AP

    IHC staining of rat stomach using 23875-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 23875-1-AP (SNRNP200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRNP200 antibody included
  • FC experiment of A431 using 23875-1-AP

    FC experiment of A431 using 23875-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human SNRNP200 (23875-1-AP) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue, mouse bladder tissue, mouse testis tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SNRNP200 Ready-To-Use IHC Kit. SNRNP200 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23875-1-AP targets SNRNP200 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SNRNP200 fusion protein Ag20748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein 200kDa (U5)
Synonyms KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1
Calculated Molecular Weight 494 aa, 57 kDa
Observed Molecular Weight 200-245 kDa
GenBank Accession NumberBC001417
Gene Symbol SNRNP200
Gene ID (NCBI) 23020
RRIDAB_2879346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO75643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.

实验方案

Product Specific Protocols
WB protocol for SNRNP200 antibody 23875-1-APDownload protocol
IHC protocol for SNRNP200 antibody 23875-1-APDownload protocol
IP protocol for SNRNP200 antibody 23875-1-APDownload protocol
FC protocol for SNRNP200 antibody 23875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
View Article
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanIP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen
View Article
humanWB

Cell Rep

SART3 reads methylarginine-marked glycine- and arginine-rich motifs

Authors - Yalong Wang
View Article