验证数据展示
经过测试的应用
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PE-23875 targets SNRNP200 in FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | FC (Intra) Application Description |
经测试反应性 | human, mouse |
免疫原 | SNRNP200 fusion protein Ag20748 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | small nuclear ribonucleoprotein 200kDa (U5) |
别名 | KIAA0788, HELIC2, EC:3.6.4.13, BRR2, ASCC3L1 |
计算分子量 | 494 aa, 57 kDa |
观测分子量 | 200-245 kDa |
GenBank蛋白编号 | BC001417 |
基因名称 | SNRNP200 |
Gene ID (NCBI) | 23020 |
RRID | AB_3673989 |
偶联类型 | PE Fluorescent Dye |
最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75643 |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.
实验方案
Product Specific Protocols | |
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FC protocol for PE SNRNP200 antibody PE-23875 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |