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SNRPN/SNRPB Monoclonal antibody, PBS Only

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2F4

Cat no : 67280-1-PBS

Print datasheet

Synonyms

SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

验证数据展示

  • WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)

    WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)

    IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

产品信息

67280-1-PBS targets SNRPN/SNRPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNRPN/SNRPB fusion protein Ag14030 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide N
Synonyms SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC000611
Gene Symbol SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP63162
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.