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验证数据展示

  • WB analysis using 67280-1-Ig

    WB analysis using 67280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 67280-1-Ig

    IF Staining of SH-SY5Y using 67280-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SNRPN/SNRPB Monoclonal antibody

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67280-1-Ig

产品说明书

CloneNo. 1H2F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67280-1-Ig targets SNRPN/SNRPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNRPN/SNRPB fusion protein Ag14030 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide N
别名 SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D
计算分子量 240 aa, 25 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC000611
基因名称 SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP63162
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.

实验方案

Product Specific Protocols
WB protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
IHC protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
IF protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols