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验证数据展示

  • WB analysis using 67280-1-Ig

    WB analysis using 67280-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67280-1-Ig

    IHC staining of human colon cancer using 67280-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 67280-1-Ig

    IF Staining of SH-SY5Y using 67280-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SNRPN/SNRPB Monoclonal antibody

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67280-1-Ig

Print datasheet

CloneNo. 1H2F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67280-1-Ig targets SNRPN/SNRPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNRPN/SNRPB fusion protein Ag14030 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide N
Synonyms SNRPN, SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC000611
Gene Symbol SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP63162
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.

实验方案

Product Specific Protocols
WB protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
IHC protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
IF protocol for SNRPN/SNRPB antibody 67280-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols