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验证数据展示

  • FC experiment of HeLa using CL488-67280

    FC experiment of HeLa using CL488-67280

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SNRPN (CL488-67280, Clone:1H2F4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U-251 using CL488-67280

    IF Staining of U-251 using CL488-67280

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SNRPN antibody (CL488-67280, Clone: 1H2F4 ) at dilution of 1:1000, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SNRPN Monoclonal antibody

SNRPN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67280

产品说明书

CloneNo. 1H2F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67280-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67280 targets SNRPN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SNRPN fusion protein Ag14030 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide N
别名 SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3
计算分子量 240 aa, 25 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC000611
基因名称 SNRPN
Gene ID (NCBI) 6638
RRIDAB_2923767
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP63162
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
FC protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
Standard Protocols
Click here to view our Standard Protocols