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验证数据展示

  • FC experiment of HeLa using CL488-67280

    FC experiment of HeLa using CL488-67280

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SNRPN (CL488-67280, Clone:1H2F4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U-251 using CL488-67280

    IF Staining of U-251 using CL488-67280

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SNRPN antibody (CL488-67280, Clone: 1H2F4 ) at dilution of 1:1000, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SNRPN Monoclonal antibody

SNRPN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67280

Print datasheet

CloneNo. 1H2F4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67280-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67280 targets SNRPN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SNRPN fusion protein Ag14030 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide N
Synonyms SMN, Sm-D, Small nuclear ribonucleoprotein-associated protein N, SM D, HCERN3
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC000611
Gene Symbol SNRPN
Gene ID (NCBI) 6638
RRIDAB_2923767
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP63162
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
FC protocol for CL Plus 488 SNRPN antibody CL488-67280Download protocol
Standard Protocols
Click here to view our Standard Protocols