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Species Reactivity
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验证数据展示

  • WB analysis using 68305-1-Ig

    WB analysis using 68305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68305-1-Ig

    IF Staining of A431 using 68305-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68305-1-Ig

Print datasheet

CloneNo. 2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68305-1-Ig targets SNX18 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen SNX18 fusion protein Ag17388 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting nexin 18
Synonyms FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
RRIDAB_2935384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96RF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
WB protocol for SNX18 antibody 68305-1-IgDownload protocol
IF protocol for SNX18 antibody 68305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols