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验证数据展示

  • IF Staining of A431 using CL488-68305

    IF Staining of A431 using CL488-68305

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 SNX18 antibody (CL488-68305, Clone: 2E7D6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for IF/ICC
Cat No. CL488-68305

Print datasheet

CloneNo. 2E7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF/ICC

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68305-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68305 targets SNX18 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse
Immunogen SNX18 fusion protein Ag17388 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting nexin 18
Synonyms FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18
Calculated Molecular Weight 628 aa, 69 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
RRIDAB_3084470
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96RF0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNX18 antibody CL488-68305Download protocol
Standard Protocols
Click here to view our Standard Protocols