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验证数据展示

  • IF Staining of A431 using CL488-68305

    IF Staining of A431 using CL488-68305

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 SNX18 antibody (CL488-68305, Clone: 2E7D6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for IF/ICC
Cat No. CL488-68305

产品说明书

CloneNo. 2E7D6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

IF/ICC

别名

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68305-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68305 targets SNX18 in IF/ICC applications and shows reactivity with Human, Mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse
免疫原 SNX18 fusion protein Ag17388 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 18
别名 FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18
计算分子量 628 aa, 69 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC060791
基因名称 SNX18
Gene ID (NCBI) 112574
RRIDAB_3084470
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96RF0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SNX18 antibody CL488-68305Download protocol
Standard Protocols
Click here to view our Standard Protocols