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SNX27 Monoclonal antibody

SNX27 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4G5

Cat no : 68386-1-Ig

Print datasheet

Synonyms

Sorting nexin-27, My014, KIAA0488, 2C4G5

验证数据展示

  • WB analysis using 68386-1-Ig

    WB analysis using 68386-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68386-1-Ig (SNX27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68386-1-Ig

    IF Staining of HeLa using 68386-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX27 antibody (68386-1-Ig, Clone: 2C4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68386-1-Ig targets SNX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX27 fusion protein Ag9447 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting nexin family member 27
Synonyms Sorting nexin-27, My014, KIAA0488, 2C4G5
Calculated Molecular Weight 61 kDa, 60 kDa, 52 kDa, 28 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC012184
Gene Symbol SNX27
Gene ID (NCBI) 81609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96L92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes the recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).

实验方案

Product Specific Protocols
WB protocol for SNX27 antibody 68386-1-IgDownload protocol
IF protocol for SNX27 antibody 68386-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols