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SNX5 Monoclonal antibody, PBS Only

SNX5 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

2F8A4

Cat No : 67665-1-PBS

Print datasheet

Synonyms

SNX5, Sorting nexin 5

验证数据展示

  • WB analysis using 67665-1-Ig (same clone as 67665-1-PBS)

    WB analysis using 67665-1-Ig (same clone as 67665-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67665-1-Ig (SNX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SNX5 antibody included
  • IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SNX5 antibody included
  • IF Staining of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    IF Staining of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67665-1-Ig (SNX5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    FC experiment of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SNX5 (67665-1-Ig, Clone:2F8A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SNX5 Ready-To-Use IHC Kit. SNX5 primary antibody included.

产品信息

67665-1-PBS targets SNX5 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen SNX5 fusion protein Ag12330 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 5
Synonyms SNX5, Sorting nexin 5
Calculated Molecular Weight 404 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC093623
Gene Symbol SNX5
Gene ID (NCBI) 27131
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).