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SNX5 Monoclonal antibody

SNX5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F8A4

Cat no : 67665-1-Ig

Print datasheet

Synonyms

Sorting nexin-5, 2F8A4

验证数据展示

  • WB analysis using 67665-1-Ig

    WB analysis using 67665-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67665-1-Ig (SNX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67665-1-Ig

    IHC staining of human liver cancer using 67665-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX5 antibody included
  • IHC staining of human liver cancer using 67665-1-Ig

    IHC staining of human liver cancer using 67665-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX5 antibody included
  • IF Staining of HeLa using 67665-1-Ig

    IF Staining of HeLa using 67665-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67665-1-Ig (SNX5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

  • FC experiment of HeLa using 67665-1-Ig

    FC experiment of HeLa using 67665-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SNX5 (67665-1-Ig, Clone:2F8A4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNX5 Ready-To-Use IHC Kit. SNX5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67665-1-Ig targets SNX5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX5 fusion protein Ag12330 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 5
Synonyms Sorting nexin-5, 2F8A4
Calculated Molecular Weight 404 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC093623
Gene Symbol SNX5
Gene ID (NCBI) 27131
RRIDAB_2882861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

实验方案

Product Specific Protocols
WB protocol for SNX5 antibody 67665-1-IgDownload protocol
IHC protocol for SNX5 antibody 67665-1-IgDownload protocol
IF protocol for SNX5 antibody 67665-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols