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验证数据展示

  • FC experiment of HeLa using CL488-67665

    FC experiment of HeLa using CL488-67665

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SNX5 (CL488-67665, Clone:2F8A4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SNX5 Monoclonal antibody

SNX5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67665

产品说明书

CloneNo. 2F8A4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

Sorting nexin-5, 2F8A4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNX5 Ready-To-Use IHC Kit. SNX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67665-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67665 targets SNX5 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 SNX5 fusion protein Ag12330 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 5
别名 Sorting nexin-5, 2F8A4
计算分子量 404 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC093623
基因名称 SNX5
Gene ID (NCBI) 27131
RRIDAB_3084370
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5X3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SNX5 antibody CL488-67665Download protocol
Standard Protocols
Click here to view our Standard Protocols