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验证数据展示

  • FC experiment of HeLa using CL488-67665

    FC experiment of HeLa using CL488-67665

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SNX5 (CL488-67665, Clone:2F8A4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SNX5 Monoclonal antibody

SNX5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67665

Print datasheet

CloneNo. 2F8A4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Synonyms

Sorting nexin-5, 2F8A4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SNX5 Ready-To-Use IHC Kit. SNX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67665-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67665 targets SNX5 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen SNX5 fusion protein Ag12330 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sorting nexin 5
Synonyms Sorting nexin-5, 2F8A4
Calculated Molecular Weight 404 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC093623
Gene Symbol SNX5
Gene ID (NCBI) 27131
RRIDAB_3084370
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SNX5 antibody CL488-67665Download protocol
Standard Protocols
Click here to view our Standard Protocols