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  • KD/KO Validated

SOCS3 Monoclonal antibody

SOCS3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF-P, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1G2B12

Cat no : 66797-1-Ig

Print datasheet

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3



经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, HeLa cells, Ramos cells, CTLL-2 cells, pig spleen tissue
Positive IHC detected inhuman breast cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66797-1-Ig targets SOCS3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen SOCS3 fusion protein Ag5386 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name suppressor of cytokine signaling 3
Synonyms ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC060858
Gene Symbol SOCS3
Gene ID (NCBI) 9021
RRIDAB_2882141
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).

实验方案

Product Specific Protocols
WB protocol for SOCS3 antibody 66797-1-IgDownload protocol
IHC protocol for SOCS3 antibody 66797-1-IgDownload protocol
IF protocol for SOCS3 antibody 66797-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC

Acta Pharmacol Sin

Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.

Authors - Wen-Xia Yang
  • KD Validated
mouseWB

Int J Mol Sci

Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS).

Authors - Agueda Ferrer-Donato
ratWB, IP

Int J Mol Sci

Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis.

Authors - Vicente Barrios
mouseWB

J Ethnopharmacol

Simiao San alleviates hyperuricemia and kidney inflammation by inhibiting NLRP3 inflammasome and JAK2/STAT3 signaling in hyperuricemia mice

Authors - Yueyi Zhang
mouseWB

Mol Nutr Food Res

Single Treatment of Vitamin D3 Ameliorates LPS-Induced Acute Lung Injury through Changing Lung Rodentibacter abundance.

Authors - Ai Jin
mouseWB

Front Cell Dev Biol

Autophagy Drives Galectin-1 Secretion From Tumor-Associated Macrophages Facilitating Hepatocellular Carcinoma Progression.

Authors - Goutham Venkata Naga Davuluri