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  • Featured Product
  • KD/KO Validated

SOCS3 Monoclonal antibody, PBS Only

SOCS3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G2B12

Cat No : 66797-1-PBS

Print datasheet

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3

验证数据展示

  • IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    IHC staining of human breast cancer using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

    IF Staining of mouse heart using 66797-1-Ig (same clone as 66797-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SOCS3 antibody (66797-1-Ig, Clone: 1G2B12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66797-1-Ig (same clone as 66797-1-PBS)

    WB analysis of HeLa using 66797-1-Ig (same clone as 66797-1-PBS)

    WB result of SOCS3 antibody (66797-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

  • WB analysis using 66797-1-Ig (same clone as 66797-1-PBS)

    WB analysis using 66797-1-Ig (same clone as 66797-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66797-1-PBS in a different storage buffer formulation.

产品信息

66797-1-PBS targets SOCS3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse
Immunogen SOCS3 fusion protein Ag5386 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name suppressor of cytokine signaling 3
Synonyms ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC060858
Gene Symbol SOCS3
Gene ID (NCBI) 9021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14543
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).