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  • Featured Product
  • KD/KO Validated

SOD1 Monoclonal antibody, PBS Only

SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10G1

Cat no : 67480-1-PBS

Print datasheet

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

验证数据展示

  • WB analysis using 67480-1-Ig (same clone as 67480-1-PBS)

    WB analysis using 67480-1-Ig (same clone as 67480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67480-1-Ig (SOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67480-1-Ig (same clone as 67480-1-PBS)

    WB analysis of HeLa using 67480-1-Ig (same clone as 67480-1-PBS)

    HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 67480-1-Ig incubated at 1:10000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

    IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOD1 antibody included
  • IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

    IHC staining of human liver cancer using 67480-1-Ig (same clone as 67480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67480-1-Ig (SOD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOD1 antibody included
  • IF Staining of NIH/3T3 using 67480-1-Ig (same clone as 67480-1-PBS)

    IF Staining of NIH/3T3 using 67480-1-Ig (same clone as 67480-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using SOD1 antibody (67480-1-Ig, Clone: 2F10G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 67480-1-Ig (same clone as 67480-1-PBS)

    FC experiment of HEK-293T using 67480-1-Ig (same clone as 67480-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human SOD1 (67480-1-Ig, Clone:2F10G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67480-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

产品信息

67480-1-PBS targets SOD1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity rabbit, pig, rat, mouse, human
Immunogen SOD1 fusion protein Ag28553 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC001034
Gene Symbol SOD1
Gene ID (NCBI) 6647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00441
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643). SOD1 can be detected 24, 32, 40 and 48 kDa by ubiquitination (PMID: 16943203).