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SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2F10G1

Cat no : 67480-1-Ig

Print datasheet

Synonyms

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1



经过测试的应用

Positive WB detected inHeLa cells, rabbit brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HEK-293T cells
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67480-1-Ig targets SOD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, pig
Immunogen SOD1 fusion protein Ag28553 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC001034
Gene Symbol SOD1
Gene ID (NCBI) 6647
RRIDAB_2882707
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643). SOD1 can be detected 24, 32, 40 and 48 kDa by ubiquitination (PMID: 16943203).

实验方案

Product Specific Protocols
WB protocol for SOD1 antibody 67480-1-IgDownload protocol
IHC protocol for SOD1 antibody 67480-1-IgDownload protocol
IF protocol for SOD1 antibody 67480-1-IgDownload protocol
FC protocol for SOD1 antibody 67480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Oxid Med Cell Longev

Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2

Authors - Jie Jin
ratWB

Oxid Med Cell Longev

Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.

Authors - Honglin Liu
humanWB

J Agric Food Chem

Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition

Authors - Ji-Wang Deng
mouseWB

Diabetes Metab J

Pharmacologic Activation of Angiotensin-Converting Enzyme II Alleviates Diabetic Cardiomyopathy in db/db Mice by Reducing Reactive Oxidative Stress

Authors - Donghyun Kim
mouseWB

Phytomedicine

β-Lapachone, an NQO1 activator, alleviates diabetic cardiomyopathy by regulating antioxidant ability and mitochondrial function.

Authors - Pei-Yu Wu
mouseWB

Front Pharmacol

Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury.

Authors - Haoyang Ma