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SOD1 Monoclonal antibody
SOD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2F10G1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, rabbit brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, K-562 cells, HSC-T6 cells |
Positive IHC detected in | human liver cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NIH/3T3 cells, HEK-293T cells |
Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67480-1-Ig targets SOD1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | SOD1 fusion protein Ag28553 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | superoxide dismutase 1, soluble |
Synonyms | IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1 |
Calculated Molecular Weight | 16 kDa |
Observed Molecular Weight | 16-20 kDa |
GenBank Accession Number | BC001034 |
Gene Symbol | SOD1 |
Gene ID (NCBI) | 6647 |
RRID | AB_2882707 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00441 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SOD1 antibody 67480-1-Ig | Download protocol |
IHC protocol for SOD1 antibody 67480-1-Ig | Download protocol |
IF protocol for SOD1 antibody 67480-1-Ig | Download protocol |
FC protocol for SOD1 antibody 67480-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
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Oxid Med Cell Longev Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2 | ||
Oxid Med Cell Longev Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis. | ||
J Agric Food Chem Mechanism of Porphyra Yezoensis Polysaccharides in Inhibiting Hyperoxalate-Induced Renal Injury and Crystal Deposition | ||
Diabetes Metab J Pharmacologic Activation of Angiotensin-Converting Enzyme II Alleviates Diabetic Cardiomyopathy in db/db Mice by Reducing Reactive Oxidative Stress | ||
Phytomedicine β-Lapachone, an NQO1 activator, alleviates diabetic cardiomyopathy by regulating antioxidant ability and mitochondrial function. | ||
Front Pharmacol Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury. |