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验证数据展示

  • FC experiment of HEK-293T using CL594-67480

    FC experiment of HEK-293T using CL594-67480

    1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOD1 (CL594-67480, Clone:2F10G1) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67480

产品说明书

CloneNo. 2F10G1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

FC (Intra)

别名

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67480-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67480 targets SOD1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 SOD1 fusion protein Ag28553 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 superoxide dismutase 1, soluble
别名 IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1
计算分子量 16 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC001034
基因名称 SOD1
Gene ID (NCBI) 6647
RRIDAB_3084771
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00441
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
FC protocol for CL594 SOD1 antibody CL594-67480Download protocol
Standard Protocols
Click here to view our Standard Protocols