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CoraLite®594-conjugated SOD1 Monoclonal antibody

SOD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F10G1

Cat No : CL594-67480

Print datasheet

Synonyms

ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

验证数据展示

  • FC experiment of HEK-293T using CL594-67480

    FC experiment of HEK-293T using CL594-67480

    1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOD1 (CL594-67480, Clone:2F10G1) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67480-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67480 targets SOD1 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen SOD1 fusion protein Ag28553 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC001034
Gene Symbol SOD1
Gene ID (NCBI) 6647
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00441
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
FC protocol for CL594 SOD1 antibody CL594-67480Download protocol
Standard Protocols
Click here to view our Standard Protocols