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验证数据展示

  • IF Staining of HepG2 using 83519-2-RR (same clone as 83519-2-PBS)

    IF Staining of HepG2 using 83519-2-RR (same clone as 83519-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    IF Staining of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    FC experiment of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-2-RR, Clone:240462F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00517-1

    Cytometric bead array standard curve of MP00517-1

    Cytometric bead array standard curve of MP00517-1, SOD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83519-2-PBS. Detection antibody: 83519-1-PBS. Standard: Ag21388. Range: 1.56-200 ng/mL.

  • Affinity and Kinetic Characterization of 83519-2-RR

    Affinity and Kinetic Characterization of 83519-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83519-2-RR against Human SOD2 were performed. The affinity constant is 2.75 nM.

SOD2 Recombinant antibody, PBS Only (Capture)

SOD2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83519-2-PBS

产品说明书

CloneNo. 240462F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

SODM, MNSOD, Mn SOD, IPOB, IPO B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.

产品信息

83519-2-PBS targets SOD2 as part of a matched antibody pair:

MP00517-1: 83519-2-PBS capture and 83519-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SOD2 fusion protein Ag21388 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 superoxide dismutase 2, mitochondrial
别名 SODM, MNSOD, Mn SOD, IPOB, IPO B
计算分子量 25 kDa
GenBank蛋白编号BC016934
基因名称 SOD2
Gene ID (NCBI) 6648
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04179
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.