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SOD2 Recombinant antibody

SOD2 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240462F8

Cat No : 83519-2-RR

Print datasheet

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM

验证数据展示

  • IF Staining of HepG2 using 83519-2-RR

    IF Staining of HepG2 using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83519-2-RR

    IF Staining of HeLa using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83519-2-RR is SOD2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen SOD2 fusion protein Ag21388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name superoxide dismutase 2, mitochondrial
Synonyms IPO B, Mn SOD, MNSOD, SOD2, SODM
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.

实验方案

Product Specific Protocols
IF protocol for SOD2 antibody 83519-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols