• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23002-1-AP

Print datasheet

Synonyms

VPS10 domain-containing receptor SorCS1, SORCS, hSorCS

验证数据展示

  • WB analysis using 23002-1-AP

    WB analysis using 23002-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 23002-1-AP

    IHC staining of human brain using 23002-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 23002-1-AP

    IHC staining of human brain using 23002-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of HeLa using 23002-1-AP

    FC experiment of HeLa using 23002-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SORCS1 Polyclonal antibody (23002-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, human brain tissue, Y79 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23002-1-AP targets SORCS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SORCS1 fusion protein Ag19199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sortilin-related VPS10 domain containing receptor 1
Synonyms VPS10 domain-containing receptor SorCS1, SORCS, hSorCS
Calculated Molecular Weight 1168 aa, 130 kDa
Observed Molecular Weight 60-80 kDa
GenBank Accession NumberBC131597
Gene Symbol SORCS1
Gene ID (NCBI) 114815
RRIDAB_2879196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8WY21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SORCS1, also named as SorCS, belongs to the family of VPS10 domain-containing receptors. SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for SORCS1 antibody 23002-1-APDownload protocol
IHC protocol for SORCS1 antibody 23002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression.

Authors - Junfen Xu
View Article