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SOX10 Polyclonal antibody, PBS Only

SOX10 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10422-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse brain using 10422-1-AP (same clone as 10422-1-PBS)

    WB analysis of mouse brain using 10422-1-AP (same clone as 10422-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10422-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse colon using 10422-1-AP (same clone as 10422-1-PBS)

    IP experiment of mouse colon using 10422-1-AP (same clone as 10422-1-PBS)

    IP result of anti-SOX10 (IP:10422-1-AP, 4ug; Detection:10422-1-AP 1:2000) with mouse colon tissue lysate 4000 ug. This data was developed using the same antibody clone with 10422-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 10422-1-AP (same clone as 10422-1-PBS)

    IHC staining of human brain using 10422-1-AP (same clone as 10422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10422-1-AP (SOX10 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10422-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX10 antibody included
  • FC experiment of C6 using 10422-1-AP (same clone as 10422-1-PBS)

    FC experiment of C6 using 10422-1-AP (same clone as 10422-1-PBS)

    1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (10422-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 10422-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

产品信息

10422-1-PBS targets SOX10 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SOX10 fusion protein Ag0673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 10
Synonyms
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2195371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56693
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)