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验证数据展示

  • WB analysis of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    WB analysis of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66786-1-Ig (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    IF Staining of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX10 antibody included
  • FC experiment of C6 using 66786-1-Ig (same clone as 66786-1-PBS)

    FC experiment of C6 using 66786-1-Ig (same clone as 66786-1-PBS)

    1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (66786-1-Ig, Clone:1D2C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66786-1-Ig (same clone as 66786-1-PBS)

    IHC staining of rat cerebellum using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX10 antibody included
  • IF Staining of rat brain using 66786-1-Ig (same clone as 66786-1-PBS)

    IF Staining of rat brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50275-1

    Cytometric bead array standard curve of MP50275-1

    Cytometric bead array standard curve of MP50275-1, SOX10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66786-1-PBS. Detection antibody: 66786-2-PBS. Standard:Ag23191. Range: 1.563-100 ng/mL.

SOX10 Monoclonal antibody, PBS Only (Capture)

SOX10 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66786-1-PBS

产品说明书

CloneNo. 1D2C8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

产品信息

66786-1-PBS targets SOX10 as part of a matched antibody pair:

MP50275-1: 66786-1-PBS capture and 66786-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SOX10 fusion protein Ag23191 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM
计算分子量 50 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
RRIDAB_2882131
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56693
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)