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SOX10 Monoclonal antibody, PBS Only

SOX10 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat No : 66786-1-PBS

Print datasheet

Synonyms

DOM, SOX10, Transcription factor SOX 10, WS2E, WS4

验证数据展示

  • WB analysis of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    WB analysis of A375 using 66786-1-Ig (same clone as 66786-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66786-1-Ig (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    IHC staining of human malignant melanoma using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SOX10 antibody included
  • FC experiment of C6 using 66786-1-Ig (same clone as 66786-1-PBS)

    FC experiment of C6 using 66786-1-Ig (same clone as 66786-1-PBS)

    1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (66786-1-Ig, Clone:1D2C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 66786-1-Ig (same clone as 66786-1-PBS)

    IF Staining of rat brain using 66786-1-Ig (same clone as 66786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66786-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.

产品信息

66786-1-PBS targets SOX10 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, mouse, rat
Immunogen SOX10 fusion protein Ag23191 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 10
Synonyms DOM, SOX10, Transcription factor SOX 10, WS2E, WS4
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56693
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)