SOX10 Monoclonal antibody
SOX10 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1D2C8
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells |
| Positive IHC detected in | human malignant melanoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive FC (Intra) detected in | C6 cells |
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66786-1-Ig targets SOX10 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, chicken |
| Immunogen | SOX10 fusion protein Ag23191 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SRY (sex determining region Y)-box 10 |
| Synonyms | Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC002824 |
| Gene Symbol | SOX10 |
| Gene ID (NCBI) | 6663 |
| RRID | AB_2882131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56693 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IHC protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| IF protocol for SOX10 antibody 66786-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve. | ||
Physiol Behav Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria. | ||
J Neurosci Methods Characterization of the GfaABC1D promoter to selectively target astrocytes in the rhesus macaque brain. | ||
Res Sq Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries | ||
