SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat no : 66786-1-Ig

Print datasheet

Synonyms

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM



经过测试的应用

Positive WB detected inA375 cells
Positive IHC detected inhuman malignant melanoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse brain tissue
Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66786-1-Ig targets SOX10 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, chicken
Immunogen SOX10 fusion protein Ag23191 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 10
Synonyms Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2882131
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56693
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
WB protocol for SOX10 antibody 66786-1-IgDownload protocol
IHC protocol for SOX10 antibody 66786-1-IgDownload protocol
IF protocol for SOX10 antibody 66786-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Elife

Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve.

Authors - Sonia Taïb
chickenIF

Front Physiol

Cadherin-11 Is Required for Neural Crest Specification and Survival.

Authors - Subrajaa Manohar
ratIF

Physiol Behav

Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria.

Authors - Carolina R Cawthon
IF

J Neurosci Methods

Characterization of the GfaABC1D promoter to selectively target astrocytes in the rhesus macaque brain.

Authors - Kate S Heffernan
rabbitWB

Anim Biosci

Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits

Authors - Yang Chen
rabbitIHC

Res Sq

Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries

Authors - Mario RomeroOrtega