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CoraLite®594-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D2C8

Cat no : CL594-66786

Print datasheet

Synonyms

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

验证数据展示

  • IF Staining of mouse brain using CL594-66786

    IF Staining of mouse brain using CL594-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594-conjugated SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:100.

  • IF Staining of mouse brain using CL594-66786

    IF Staining of mouse brain using CL594-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200. DAPI (blue).

  • IF Staining of rat brain using CL594-66786

    IF Staining of rat brain using CL594-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • IF Staining of rat brain using CL594-66786

    IF Staining of rat brain using CL594-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • FC experiment of C6 using CL594-66786

    FC experiment of C6 using CL594-66786

    1X10^6 C6 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOX10 (CL594-66786, Clone:1D2C8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse brain tissue, rat brain tissue
Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66786-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66786 targets SOX10 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SOX10 fusion protein Ag23191 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 10
Synonyms Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM
Calculated Molecular Weight 50 kDa
Observed Molecular Weight
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2920037
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56693
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
IF protocol for CL594 SOX10 antibody CL594-66786Download protocol
FC protocol for CL594 SOX10 antibody CL594-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols