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CoraLite®555-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1D2C8

Cat no : CL555-66786

Print datasheet

Synonyms

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

验证数据展示

  • FC experiment of C6 using CL555-66786

    FC experiment of C6 using CL555-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOX10 (CL555-66786, Clone:1D2C8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66786-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66786 targets SOX10 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen SOX10 fusion protein Ag23191 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 10
Synonyms Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56693
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
FC protocol for CL555 SOX10 antibody CL555-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols