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验证数据展示

  • FC experiment of C6 using CL555-66786

    FC experiment of C6 using CL555-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOX10 (CL555-66786, Clone:1D2C8) (red). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of A375 using CL555-66786

    FC experiment of A375 using CL555-66786

    1X10^6 A375 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOX10 (CL555-66786, Clone:1D2C8) (red). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®555-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for FC (Intra)
Cat No. CL555-66786

产品说明书

CloneNo. 1D2C8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

1D2C8, DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inC6 cells, A375 cells
Planning an IHC experiment? We recommend our IHCeasy SOX10 Ready-To-Use IHC Kit. SOX10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66786-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66786 targets SOX10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SOX10 fusion protein Ag23191 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 10
别名 1D2C8, DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10
计算分子量 50 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC002824
基因名称 SOX10
Gene ID (NCBI) 6663
RRIDAB_2934678
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56693
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

实验方案

Product Specific Protocols
FC protocol for CL555 SOX10 antibody CL555-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols