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SP1 Polyclonal antibody

SP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 21962-1-AP

Print datasheet

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1



经过测试的应用

Positive WB detected inJurkat cells, A431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21962-1-AP targets SP1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications chIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, monkey, pig
Immunogen SP1 fusion protein Ag16720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sp1 transcription factor
Synonyms SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-106 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
RRIDAB_10898171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

实验方案

Product Specific Protocols
WB protocol for SP1 antibody 21962-1-APDownload protocol
IHC protocol for SP1 antibody 21962-1-APDownload protocol
IF protocol for SP1 antibody 21962-1-APDownload protocol
IP protocol for SP1 antibody 21962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
mouseWB

Sci Adv

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Authors - Xiaoqun Li
humanWB

Nucleic Acids Res

High mobility group AT-hook 1 (HMGA1) is an important positive regulator of hepatitis B virus (HBV) that is reciprocally upregulated by HBV X protein.

Authors - Zhongliang Shen
mouseWB

Drug Des Devel Ther

Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways

Authors - Li-Shan Yan
mouseWB

EMBO J

AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

Authors - Xin Zheng
humanWB

Cancer Lett

Pleckstrin-2 promotes tumour immune escape from NK cells by activating the MT1-MMP-MICA signalling axis in gastric cancer

Authors - Deli Mao