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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SP1 Polyclonal antibody

SP1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-21962

Print datasheet

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

验证数据展示

  • IF Staining of HepG2 using CL594-21962

    IF Staining of HepG2 using CL594-21962

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SP1 antibody (CL594-21962) at dilution of 1:200.

  • FC experiment of HeLa using CL594-21962

    FC experiment of HeLa using CL594-21962

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SP1 (CL594-21962) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21962-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-21962 is SP1 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat, monkey
Immunogen SP1 fusion protein Ag16720 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sp1 transcription factor
Synonyms SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-106 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
RRIDAB_2919865
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08047
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

实验方案

Product Specific Protocols
IF protocol for CL594 SP1 antibody CL594-21962Download protocol
FC protocol for CL594 SP1 antibody CL594-21962Download protocol
Standard Protocols
Click here to view our Standard Protocols