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CoraLite®594-conjugated SP1 Polyclonal antibody
SP1 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21962-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-21962 targets SP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, monkey |
Immunogen | SP1 fusion protein Ag16720 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Sp1 transcription factor |
Synonyms | TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1 |
Calculated Molecular Weight | 785 aa, 81 kDa |
Observed Molecular Weight | 95-106 kDa |
GenBank Accession Number | BC062539 |
Gene Symbol | SP1 |
Gene ID (NCBI) | 6667 |
ENSEMBL Gene ID | ENSG00000185591 |
RRID | AB_2919865 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08047 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 SP1 antibody CL594-21962 | Download protocol |
FC protocol for CL594 SP1 antibody CL594-21962 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |