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验证数据展示

  • WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

SPAG11A/B Polyclonal antibody, PBS Only

SPAG11A/B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16770-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

SPAG11A, Antimicrobial-like protein Bin-1b, BIN1B, EP2, He2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16770-1-PBS targets SPAG11A/B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SPAG11A/B fusion protein Ag9846 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 11A
别名 SPAG11A, Antimicrobial-like protein Bin-1b, BIN1B, EP2, He2
计算分子量 123 aa, 14 kDa
观测分子量 20 kDa
GenBank蛋白编号BC058833
基因名称 SPAG11A
Gene ID (NCBI) 653423
RRIDAB_2286582
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PDA7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671). SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing. This antibody recognizes SPAG11A and SPAG11B, but not SPAG11, because SPAG11 is a different transcript.