验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, human brain tissue |
| Positive IHC detected in | human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
16770-1-AP targets SPAG11A in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | SPAG11A fusion protein Ag9846 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sperm associated antigen 11A |
| 别名 | EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A |
| 计算分子量 | 123 aa, 14 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC058833 |
| 基因名称 | SPAG11A |
| Gene ID (NCBI) | 653423 |
| RRID | AB_2286582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PDA7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAG11A antibody 16770-1-AP | Download protocol |
| IHC protocol for SPAG11A antibody 16770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
