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验证数据展示

  • WB analysis using 84621-3-RR (same clone as 84621-3-PBS)

    WB analysis using 84621-3-RR (same clone as 84621-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84621-3-RR (SPARC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84621-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

  • Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

  • Affinity and Kinetic Characterization of 84621-3-RR

    Affinity and Kinetic Characterization of 84621-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84621-3-RR against Human SPARC were performed. The affinity constant is 1.55 nM.

SPARC Recombinant antibody, PBS Only (Detector)

SPARC Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84621-3-PBS

产品说明书

CloneNo. 241952E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.

产品信息

84621-3-PBS targets SPARC as part of a matched antibody pair:

MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 secreted protein, acidic, cysteine-rich (osteonectin)
别名 ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40
计算分子量35kDa
观测分子量40-43 kDa
GenBank蛋白编号NM_003118.4
基因名称 SPARC
Gene ID (NCBI) 6678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09486
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).