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验证数据展示

  • WB analysis using 84621-3-RR

    WB analysis using 84621-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84621-3-RR (SPARC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84621-3-RR

    Affinity and Kinetic Characterization of 84621-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84621-3-RR against Human SPARC were performed. The affinity constant is 1.55 nM.

SPARC Recombinant antibody

SPARC Recombinant Antibody for WB, ELISA
Cat No. 84621-3-RR

产品说明书

CloneNo. 241952E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, human testis tissue
Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84621-3-RR targets SPARC in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 secreted protein, acidic, cysteine-rich (osteonectin)
别名 ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40
计算分子量35kDa
观测分子量40-43 kDa
GenBank蛋白编号NM_003118.4
基因名称 SPARC
Gene ID (NCBI) 6678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09486
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

实验方案

Product Specific Protocols
WB protocol for SPARC antibody 84621-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols