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验证数据展示

  • IF Staining of C6 using CL488-66426

    IF Staining of C6 using CL488-66426

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite® Plus 488 SPARC antibody (CL488-66426, Clone: 1A2C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SPARC Monoclonal antibody

SPARC Monoclonal Antibody for IF/ICC
Cat No. CL488-66426

Print datasheet

CloneNo. 1A2C1

Host / Isotype

Mouse / IgG2a

Reactivity

human, Rat

Applications

IF/ICC

Synonyms

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inC6 cells
Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66426-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66426 targets SPARC in IF/ICC applications and shows reactivity with human, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, Rat
Immunogen SPARC fusion protein Ag7390 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Synonyms Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 38 kDa, 52 kDa
GenBank Accession NumberBC004974
Gene Symbol SPARC
Gene ID (NCBI) 6678
RRIDAB_3672912
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09486
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SPARC antibody CL488-66426Download protocol
Standard Protocols
Click here to view our Standard Protocols