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  • Featured Product
  • KD/KO Validated

SPCS1 Polyclonal antibody

SPCS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 11847-1-AP

Print datasheet

Synonyms

SPC12, SPC1, SPase 12 kDa subunit, HSPC033

验证数据展示

  • WB analysis using 11847-1-AP

    WB analysis using 11847-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 11847-1-AP

    IF Staining of BxPC-3 using 11847-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11847-1-AP (SPCS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, human skin tissue, PC-3 cells
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11847-1-AP targets SPCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SPCS1 fusion protein Ag2407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal peptidase complex subunit 1 homolog (S. cerevisiae)
Synonyms SPC12, SPC1, SPase 12 kDa subunit, HSPC033
Calculated Molecular Weight 102 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000884
Gene Symbol SPCS1
Gene ID (NCBI) 28972
RRIDAB_2195400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6A9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPCS1 antibody 11847-1-APDownload protocol
IF protocol for SPCS1 antibody 11847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Authors - Rong Zhang
  • KO Validated
View Article
humanWB

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R
  • KD Validated
View Article
WB

PLoS Pathog

SPCS1-Dependent E2-p7 processing determines HCV Assembly efficiency.

Authors - Nabeel Alzahrani
View Article
humanWB

Mol Cell

Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway

Authors - Iqbal Dulloo
View Article